|
|
|
| Registros recuperados: 19 | |
|
|
| Freire,Isabel Maria Alexandre; Varges,Renato; Lilenbaum,Walter. |
| Foram testadas 120 amostras séricas de cães com títulos (<FONT FACE=Symbol>³</FONT>100) perante Leptospira spp. serovar Icterohaemorrhagiae para a determinação da atividade sérica de alanina aminotransferase (ALT), aspartato aminotransferase (AST), fosfatase alcalina (FA), gamaglutamiltransferase (GGT) e dos níveis séricos de colesterol total, triglicerídeos, proteína total, albumina, globulinas e bilirrubinas total, direta e indireta. Um grupo de 34 animais sem qualquer sintomatologia clínica e idade semelhante ao grupo testado foi utilizado como controle, tendo sido submetido às mesmas dosagens bioquímicas. Os animais com títulos <FONT FACE=Symbol>³</FONT>200 apresentaram dosagens de FA e bilirubina direta superiores ao grupo... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirose; Cães; Icterohaemorrhagiae; Fígado. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782008000900037 |
| |
|
| |
|
| |
|
| |
|
| |
|
| |
|
|
| Lilenbaum,Walter; Morais,Zenaide M.; Gonçales,Amane Paldês; Souza,Gisele O. de; Richtzenhain,Leonardo; Vasconcellos,Silvio A.. |
| Leptospires have never been recovered from goats in Brazil. Serum samples were obtained from 248 goats from Rio de Janeiro and from the seroreactive animals, urine samples were collected and processed for Leptospira isolation. A total of 52 positive reactions were observed, corresponding to 20.9% of the samples. The most prevalent reactions were to serovars Hardjo (36.5%), Shermani (30.8%), Icterohaemorrhagiae (9.6%), Grippotyphosa (9.6%), Autumnalis (5.8%), Castellonis (3.8%) and Bratislava (3.8%). Two strains of Leptospira sp. were isolated, both in the same region, but from different flocks. Presumptive identification based on serologic methods suggests those strains to be from Grippotyphosa serogroup. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goats; Leptospirosis; Leptospira sp. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300023 |
| |
|
|
| Figueiredo,Eduardo Eustáquio de Souza; Silvestre,Flávia Galindo; Campos,Wilma Neres; Furlanetto,Leone Vinícius; Medeiros,Luciana; Lilenbaum,Walter; Fonseca,Leila Sousa; Silva,Joab Trajano; Paschoalin,Vânia Margaret Flosi. |
| Isolates from suggestive bovine tuberculosis lesions were tested by a multiplex polymerase chain reaction (m-PCR) targeting for RvD1Rv2031c and IS6110 sequences, specific for M. bovis and Mycobacterium tuberculosis complex respectively. The m-PCR successfully identified as M. bovis 88.24% of the isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycobacterium bovis; Multiplex-PCR; Bovine tuberculosis. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200004 |
| |
|
|
| Marassi,Carla Dray; Fonseca,Leila de Souza; Ristow,Paula; Ferreira,Rachel; Lilenbaum,Walter; Oelemann,Walter M.R.. |
| Paratuberculosis (Johne's disease) is a chronic enteritis in cattle caused by infection with Mycobacterium avium subsp. paratuberculosis (Map). In Brazil, few reports describe the isolation of the organism. The gold standard test for Map is its isolation from tissues or feces. Moreover, bacterial growth is slow and test results are available only after three to five months of incubation Furthermore, shedding of bacilli at levels detectable by fecal culture is irregular and does not occur during the early stages of infection, which compromises the sensitivity of this methodology. The most common immunological tests to identify Map infection are complement fixation test (CFT), agar gel immunodiffusion (AGID) and enzyme-linked immunoassay (ELISA). In Brazil,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Cattle; ELISA; Serology; Diagnosis. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000200004 |
| |
|
|
| Lilenbaum,Walter; Ferreira,Rachel; Marassi,Carla Dray; Ristow,Paula; Oelemann,Walter Martins Roland; Fonseca,Leila de Souza. |
| Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Paratuberculosis; Tuberculosis; Mycobacterium bovis; Mycobacterium avium paratuberculosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016 |
| |
|
|
| Costa,Diego Figueiredo da; Silva,Aline Ferreira da; Brasil,Arthur Willian de Lima; Loureiro,Ana Paula Pereira; Santos,Fabrine Alexandre dos; Azevedo,Sergio Santos de; Lilenbaum,Walter; Alves,Clebert José. |
| ABSTRACT: Sheep breeding is an economic activity of great value for the Brazilian livestock industry, and infectious diseases can significantly compromise its productivity. The aim of this study was to detect Leptospira spp. in mixed breed sheep from a semiarid region of the State of Paraíba, Northeast Brazil. In total, 49 blood and renal tissue samples, as well as 25 urine samples, were collected from 49 sheep in the slaughter line. The microscopic agglutination test (MAT) was used to verify the presence of anti -Leptospira spp. antibodies in blood samples. Renal tissue and urine samples were analyzed by both PCR and bacterial culture. Sero reactivity was observed in 8.2% (4/49) of animals. Three renal samples were positive for leptospiral DNA by PCR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Small ruminants; PCR; Serology; Isolation; Slaughterhouse. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000200451 |
| |
|
|
| Lilenbaum,Walter; Kremer,Frederico; Ristow,Paula; Dellagostin,Odir; Bourhy,Pascale; Hartskeerl,Rudy; Vasconcellos,Silvio. |
| Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Serogrouping; Sequencing; PFGE; Rrs. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050 |
| |
|
|
| Rocha,Bruno Ribeiro; Narduche,Lorena; Oliveira,Clara Slade; Martins,Gabriel; Lilenbaum,Walter. |
| ABSTRACT: For a long time, it has been stated that urine leptospiral shedding is intermittent, which was observed primarily by culturing. However, culturing presents serious limitations, mainly low sensitivity, and failure on detection of leptospires cannot be neglected. PCR presents several advantages, mainly higher sensitivity. The present study aimed to analyze the occurrence of intermittency on leptospiral shedding by PCR in naturally and experimentally infected animals. In this study two experiments were conducted, the first with 60 cows naturally infected from an endemic herd. The second one was conducted in three sheep experimentally infected, each one with a different strain of Leptospira (strains Copenhageni L1-130, Canicola LO-4 and Pomona... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cows; Intermittent; PCR; Sheep; Strain. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800453 |
| |
|
| |
|
|
| Medeiros,Luciana dos Santos; Marassi,Carla Dray; Figueiredo,Eduardo Eustáquio S.; Lilenbaum,Walter. |
| Bovine tuberculosis, a chronic infection in cattle caused by Mycobacterium bovis, remains an economic and public health problem for several countries. Due to its economic impact on international trade, contagious nature, and implications for human health, global programs to eradicate the disease were implemented worldwide. Those programs are based on slaughtering PPD-reactive animals. Despite the National Programs in Brazil, complete eradication has not been achieved, and the disease remains, albeit at a lower prevalence. The central purpose of this review is to address diagnostic tests for tuberculosis. Considering the course of the infection in cattle, at least two tests, ideally complementary to one another, may be necessary for an adequate diagnosis:... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Tuberculosis; Mycobacterium bovis; Diagnosis. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300002 |
| |
|
|
| Vasconcelos,Carlos Otávio de Paula; Brandão,Felipe Zandonadi; Martins,Gabriel; Penna,Bruno; Souza-Fabjan,Joanna Maria Gonçalves de; Lilenbaum,Walter. |
| ABSTRACT: This study evaluated the presence of vaginitis and the bacterial load associated with different intravaginal implants in ewes. Twenty-four Dorper and crossbred ewes were allocated into three groups and received intravaginal implant containing 0.3g progesterone (CIDR(r)), 60mg MAP or sponges without progesterone (CONTROL) for six days. Then, CIDR and MAP treated-ewes received 12.5mg dinoprost and 300IU eCG. Vaginal mucus samples were collected at four times: before device insertion, at the day of its removal, 24 and 48 hours after. The samples were cultured and the colonies were counted (CFU/mL) and identified. The results obtained from the counting of CFU mL-1 were submitted to Kruskal-Wallis H test, with P<0.05 being considered significant.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intravaginal device; Microorganism; Sheep; Vaginal infection.. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400632 |
| |
|
|
| Arashiro,Eduardo Kenji Nunes; Libonati,Hugo; Santos,Gustavo Bervian dos; Nogueira,Luiz Altamiro Garcia; Souza,Guilherme Nunes de; Lilenbaum,Walter; Brandão,Felipe Zandonadi. |
| ABSTRACT: Reproductive efficiency is one of the key elements for any milk production system to be successful. In this regard, reproductive management must be planned properly and the identification of the main reproductive disorders that affect the herd is primordial for decision-making. The aim of the present study was to identify the main reproductive problems that affect dairy herds on Rio de Janeiro (RJ), Brazil. Data regarding breed, age, reproductive disorder occurrence, and breeding system were individually collected from dairy cows raised at 25 farms located at RJ. The occurrence of reproductive disorders was reported in 37% of the studied animals. Estrous repetition (76.2%) and abortion (15.6%) were the most frequent reproductive problems... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cattle; Reproductive disorders; Repeat breeding. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700701 |
| |
|
| |
|
|
| Consalter,Angélica; Zanella,Eraldo Lourenso; Martins,Gabriel Mendes de Souza; Araujo,Hugo Libonati de; Lilenbaum,Walter; Souza,Guilherme Nunes de; Ferreira,Ana Maria Reis. |
| ABSTRACT: Leptospirosis is a zoonosis that is widely distributed in tropical countries. This infection is also associated with reproductive losses in livestock, which has a significant economic impact. The objective of this study is to investigate the seroprevalence and risk factors associated with leptospiral infection in commercial sheep flocks from the northwestern mesoregion of Rio Grande do Sul. Serum samples were analyzed in 319 sheep using the Microscopic Agglutination Test (MAT). At the time of sampling, an individual and herd questionnaire was carried out to investigate the risk factors. On an individual level, positive reactions were observed in 5.6% (18/319) of the studied sera, with Sejroe being the most prevalent serogroup. Animals >3 years... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira spp.; Sheep; MAT; Seroprevalence.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001200451 |
| |
| Registros recuperados: 19 | |
|
|
|
